Journal article

MEASUREMENT OF TSH RECEPTOR BLOCKING IMMUNOGLOBULINS USING H-3 ADENINE INCORPORATION INTO FRTL-5 AND JP09 CELLS - USE IN A CHILD WITH NEONATAL-HYPOTHYROIDISM

VP MICHELANGELI, CW POON, EE ARNUS, AG FRAUMAN, J CONNELLY, PG COLMAN

Clinical Endocrinology | BLACKWELL SCIENCE LTD | Published : 1995

Abstract

OBJECTIVE: The aim of this study was to develop an assay for the measurement of thyroid blocking antibodies (TBAb), based on the ability of patient serum to inhibit TSH stimulated 3H-cAMP production following incubation of FRTL-5 or JPO9 cells with 3H-adenine. The assay was then used to evaluate a child born with neonatal hypothyroidism. DESIGN: The levels of TBAb, TSAb (thyroid stimulating antibodies), TBII (TSH binding inhibitory antibodies), and the thyroid antibodies anti-thyroid peroxidase and thyroglobulin antibodies were measured in both mother and child over a 6-month post-natal period. PATIENT: The assay for TBAb was used to evaluate a child born with neonatal hypothyroidism whose m..

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