Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)

Cristian Koepfli; Wang Nguitragool; Natalie E Hofmann; Leanne J Robinson; Maria Ome-Kaius; Jetsumon Sattabongkot; Ingrid Felger; Ivo Mueller

Journal article

Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)

Cristian Koepfli, Wang Nguitragool, Natalie E Hofmann, Leanne J Robinson, Maria Ome-Kaius, Jetsumon Sattabongkot, Ingrid Felger, Ivo Mueller

Scientific Reports | NATURE PUBLISHING GROUP | Published : 2016

DOI: 10.1038/srep39183

University of Melbourne Researchers

Leanne Robinson Author Medical Biology (W.E.H.I.)

Ivo Mueller Author Medical Biology (W.E.H.I.)

Cristian Koepfli Author Medical Biology (W.E.H.I.)

Grants

Awarded by NHMRC

Awarded by Swiss National Science Foundation

Awarded by NIH International Centers of Excellence in Malaria Research grant

Awarded by SNF Early Postdoc Mobility Fellowship

Funding Acknowledgements

We would like to thank staff of the Papua New Guinea Institute of Medical Research and Mahidol Vivax Research Unit for help with collection of samples, and all patients and communities for their consent to participate in the field studies. We thank Nattawan Rachaphaew, Lina Lorry, Samuel Maripal and Charles Kongs for parasite counts by light microscopy, and Annie Yang for providing P. falciparum culture. This study was supported by the TransEPI consortium funded by the Bill & Melinda Gates Foundation, and an NHMRC project grant (#1021455), Swiss National Science Foundation grant (310030_134889), and NIH International Centers of Excellence in Malaria Research grant (U19 AI089686). C.K. was supported by an SNF Early Postdoc Mobility Fellowship (#P2BSP3_151880). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.