Derivation of a robust mouse mammary organoid system for studying tissue dynamics
Paul R Jamieson, Johanna F Dekkers, Anne C Rios, Nai Yang Fu, Geoffrey J Lindeman, Jane E Visvader
DEVELOPMENT | COMPANY OF BIOLOGISTS LTD | Published : 2017
Advances in stem cell research have enabled the generation of 'mini organs' or organoids that recapitulate phenotypic traits of the original biological specimen. Although organoids have been demonstrated for multiple organ systems, there are more limited options for studying mouse mammary gland formation in vitro Here, we have built upon previously described culture assays to define culture conditions that enable the efficient generation of clonal organoid structures from single sorted basal mammary epithelial cells (MECs). Analysis of Confetti-reporter mice revealed the formation of uni-colored structures and thus the clonal nature of these organoids. High-resolution 3D imaging demonstrated..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
Awarded by NHMRC
This work was supported by the Australian National Health and Medical Research Council (NHMRC) (1016701, 1085191 and 1086727); by NHMRC Independent Research Institute Infrastructure Support Scheme (IRIISS); by the Victorian State Government through Victorian Cancer Agency funding and Operational Infrastructure Support; and by the Australian Cancer Research Foundation. J. F. D. is supported by a Marie Sklodowska Curie global individual fellowship of the European Commission. A. C. R. and N. Y. F. were supported by National Breast Cancer Foundation (NBCF)/Cure Cancer Australia Foundation Fellowships; G. J. L. was supported by a NHMRC Fellowship (1078730); and J. E. V. was supported by NHMRC Fellowships (1037230, 1102742).