Journal article

New resource for population genetics studies on the Australasian intertidal brown alga, Hormosira banksii: isolation and characterization of 15 polymorphic microsatellite loci through next generation DNA sequencing

A Bellgrove, A van Rooyen, AR Weeks, JS Clark, MA Doblin, AD Miller

Journal of Applied Phycology | SPRINGER | Published : 2017

Abstract

The Australasian fucoid, Hormosira banksii, commonly known as ‘Neptune’s necklace’ or ‘bubbleweed’ is regarded as an autogenic ecosystem engineer with no functional equivalents. Population declines resulting from climate change and other anthropogenic disturbances pose significant threats to intertidal biodiversity. For effective conservation strategies, patterns of gene flow and population genetic structure across the species distribution need to be clearly understood. We developed a suite of 15 polymorphic microsatellite markers using next generation sequencing of 53–55 individuals from two sites (south-western Victoria and central New South Wales, Australia) and a replicated spatially hie..

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University of Melbourne Researchers

Grants

Funding Acknowledgements

Prue McKenzie, Thea Jacob, and Aimee Hauser are acknowledged for their preliminary work in optimizing DNA extraction in H. banksii, assessing ISSR markers for this species and preliminary microsatellite marker development. Sean Blake assisted with georeferencing samples. This research was supported by the Centre for Integrative Ecology and Faculty of Science, Engineering and Built Environment, Deakin University, and the C3-Climate Change Cluster, University of Technology Sydney.