Journal article

DEMONSTRATION OF CHLAMYDIAL RNA AND DNA DURING A CULTURE-NEGATIVE STATE

SM HOLLAND, AP HUDSON, L BOBO, JA WHITTUMHUDSON, RP VISCIDI, TC QUINN, HR TAYLOR

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 1992

Abstract

Trachoma is a common blinding disease of humans caused by ocular infections with Chlamydia trachomatis. The cynomolgus monkey is a valuable primate model for the detection, pathobiology, and treatment of this infection. We have used this model system to compare the relative ability of tissue culture, direct fluorescence cytology, a modified polymerase chain reaction, and RNA blotting to detect C. trachomatis following primary infection and reinfection over 34 weeks. Six cynomolgus monkeys were given a primary ocular chlamydia infection, and 20 weeks later they were reinoculated with the same organism. All animals showed brisk inflammatory responses to the primary infection and milder inflamm..

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