Journal article

Gamma-glutamylcysteine synthetase activity in human lung epithelial (A549) cells: Factors influencing its measurement

S Ray, NLA Misso, JC Lenzo, C Robinson, PJ Thompson

Free Radical Biology and Medicine | ELSEVIER SCIENCE INC | Published : 1999


Despite the central role of gamma-glutamylcysteine synthetase (gammaGCS) in lung antioxidant defenses, the limited studies of the activity of this enzyme in respiratory cells have produced variable results. This study has examined the factors, which may influence the measurement of gammaGCS activity in cultured human lung epithelial cells (A549). Although a source of potential error, gammaGCS activity in A549 cell extracts did not vary significantly when appropriately assayed by three different methods or after removal of the endogenous inhibitor, glutathione (GSH). However, gammaGCS activity did increase significantly during the early stages of cell proliferation (3.50 +/- 0.31 vs. 2.35 +/-..

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University of Melbourne Researchers