Plant regeneration from callus of Australian fan flower, Scaevola

Abstract

Plant regeneration from callus of fan flower, Scaevola phlebopetala F. Muell., an Australian native species of horticultural significance has been achieved. The effects of coconut milk, kinetin, 2,4-D and BAP on callus and shoot induction were investigated. Coconut milk promoted callus induction. Kinetic was not required for callus induction and 2,4-D induced a poor quality callus. BAP had a significant effect on callus induction, growth and appearance of callus. BAP also induced shoot initiation from callus produced from root and leaf explants. Shoot regeneration was achieved on a medium containing 5.0 % coconut milk, 60 g/L sucrose and 3 mg/L BAP. Shoot growth required a hormone free mediu..