CLONING, EXPRESSION AND IMMUNOLOGICAL CHARACTERIZATION OF ORY S 1, THE MAJOR ALLERGEN OF RICE POLLEN
HL XU, P THEERAKULPISUT, N GOULDING, C SUPHIOGLU, MB SINGH, PL BHALLA
GENE | ELSEVIER SCIENCE BV | Published : 1995
We have isolated and characterized a cDNA clone, Ory s 1, encoding a group-1 allergen of rice pollen. The Ory s 1 protein shows significant sequence identity to the major allergen of rye-grass pollen, Lol p 1. RNA gel blot analysis shows that the Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues tested. Southern blot analysis indicates that this clone represents a member of a small gene family in rice. Western blot analyses of total rice pollen proteins with the group-1 allergen-specific monoclonal 3A2 and IgE antibodies from grass pollen-allergic patients, revealed the presence of cross-reactive antigenic and allergenic epitopes in Ory s 1.