Journal article

DEOXYRIBONUCLEOSIDE TRIPHOSPHATE POOLS AND DIFFERENTIAL THYMIDINE SENSITIVITIES OF CULTURED MOUSE LYMPHOMA AND MYELOMA CELLS

EC REYNOLDS, AW HARRIS, LR FINCH

BIOCHIMICA ET BIOPHYSICA ACTA | ELSEVIER SCIENCE BV | Published : 1979

Abstract

The effects of various concentrations of thymidine on DNA synthesis and deoxyribonucleoside triphosphate contents of a highly thymidine-sensitive cultured mouse lymphoma cell line (WEHI-7) and a relatively resistant mouse myeloma cell line (HPC-108) have been studied by 32P-labelling techniques. DNA synthesis in the myeloma cells was inhibited by thymidine at concentrations of 10(-3) M or greater, while DNA synthesis in the lymphoma cells was inhibited by concentrations 30-fold lower, consistent with the 25-fold difference between the two cell lines in sensitivity to growth inhibition by thymidine. Thymidine caused marked elevation of the dTTP and dGTP pools, slight elevation or no change in..

View full abstract

University of Melbourne Researchers