Estimation of [32P]deoxyribonucleoside triphosphates in cell extracts using periodate treatment

Abstract

Radioisotopic labeling of nucleotides in cells before extraction, coupled with two-dimensional chromatography on PEI-cellulose1 1 Abbreviations: dNTP, deoxyribonucleoside triphosphate(s); rNTP, ribonucleoside triphosphate(s); PEI, polyethyleneimine. thin layers (1,2), has been successfully used in the estimation of rNTP, ADP, and GDP (3,4). This procedure has however been less successful for the dNTP, because the large amounts of rNTP tend to overlap the dNTP so that no system for the two-dimensional development of the chromatograms has been found to give consistently satisfactory separation of all four dNTP from contaminating rNTP. Yegian (5) overcame this difficulty by an initial oxidation..