Efficient solution‐phase synthesis of multiple O‐phosphoseryl‐containing peptides related to casein and statherin

Abstract

The multiple Ser(P)‐containing peptides, H‐Ser(P)‐Ser(P)‐Ser(P)‐Glu‐Glu‐NHMe‐TFA, H‐Asp‐Ser(P)‐Ser(P)‐Glu‐Glu‐NHMe‐TFA and H‐Glu‐Ser(P)‐Ser(P)‐Glu‐Glu‐NHMe‐TFA were prepared by the use of Boc‐Ser(PO3Ph2)‐OH in the Boc mode of solution phase peptide synthesis followed by platinum‐mediated hydrogenolytic de‐protection of the Ser(PO3Ph2)‐containing peptides. The protected peptides were assembled using the mixed anhydride coupling methods with 40% TFA/CH2C12 used for removal of the Boc group from intermediate Boc‐protected peptides. Copyright © 1992, Wiley Blackwell. All rights reserved