Journal article

CHARACTERIZATION, EXPRESSION, AND IMMUNOHISTOCHEMICAL LOCALIZATION OF 5-ALPHA-REDUCTASE IN HUMAN SKIN

V LUUTHE, Y SUGIMOTO, L PUY, Y LABRIE, IL SOLACHE, M SINGH, F LABRIE

JOURNAL OF INVESTIGATIVE DERMATOLOGY | ELSEVIER SCIENCE INC | Published : 1994

Abstract

Human skin has been shown to contain a high level of 5 alpha-reductase activity, the enzyme that catalyses the conversion of the weak androgen testosterone into dihydrotestosterone, the most potent androgen. Because two types of 5 alpha-reductase genes have been characterized in humans, we have cloned 5 alpha-reductase cDNAs from adult human keratinocyte and skin fibroblast cDNA libraries to identify and gain better knowledge of the 5 alpha-reductase expressed in normal human skin. Nucleotide sequence analysis shows that the clones obtained correspond to the type I 5 alpha-reductase. RNase protection analysis using (poly A)+ RNA obtained from human skin and prostate also confirms that type I..

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University of Melbourne Researchers