Journal article

HAPLOID AND DIPLOID EXPRESSION OF A BRASSICA-CAMPESTRIS ANTHER-SPECIFIC GENE PROMOTER IN ARABIDOPSIS AND TOBACCO

HL XU, SP DAVIES, BYH KWAN, AP OBRIEN, M SINGH, RB KNOX

MOLECULAR & GENERAL GENETICS | SPRINGER VERLAG | Published : 1993

Abstract

The anther-specific cDNA clone Bcp1 from Brassica campestris is expressed in both the haploid pollen and diploid tapetum, as shown by in situ hybridization. We have isolated Bgp1, a genomic clone homologous to Bcp1. The coding region and extensive 5' flanking sequences of Bgp1 have been sequenced, and the coding region shows 88% identity with Bcp1. RNA gel blot analysis confirmed the expression of Bgp1-specific transcripts in B. campestris pollen. A 767 bp 5' DNA fragment was fused to the reporter gene beta-glucuronidase (gus) and introduced into both Arabidopsis thaliana and Nicotiana tabacum by transformation. This 5' fragment directed high-level expression in the pollen and tapetum of tra..

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