Journal article

OVERCOMING INCOMPATIBILITY IN BRASSICA-CAMPESTRIS L BY CARBON-DIOXIDE, AND DARK FIXATION OF THE GAS BY SELF-POLLINATED AND CROSS-POLLINATED PISTILS

AS DHALIWAL, CP MALIK, MB SINGH

ANNALS OF BOTANY | ACADEMIC PRESS LTD | Published : 1981

Abstract

Supplementing pollen suspension cultures with CO2 (3-5 per cent) caused a marked increase in germination and tube growth in vitro in Brassica campestris L. cv. toria. A weakening of self-incompatibility by increased CO2 levels from 3-5 per cent was observed. The percentage of pollen tubes which penetrated the cuticle layer of stigmatic papilla cells in self-pollinated pistils was high when CO2 level was 5 per cent. Phosphoenol pyruvate (PEP) carboxylase activity was greater in the pollen germinated in 4 per cent CO2 as compared to air (0.03 per cent). A possible role of CO2 for self-recognition and control of pollen tube growth is proposed, proposed. © 1981 Annals of Botany Company.

University of Melbourne Researchers