Releasing the Genomic RNA Sequestered in the Mumps Virus Nucleocapsid.
Chelsea Severin, James R Terrell, James R Zengel, Robert Cox, Richard K Plemper, Biao He, Ming Luo
J Virol | American Society for Microbiology | Published : 2016
In a negative-strand RNA virus, the genomic RNA is sequestered inside the nucleocapsid when the viral RNA-dependent RNA polymerase uses it as the template for viral RNA synthesis. It must require a conformational change in the nucleocapsid protein (N) to make the RNA accessible to the viral polymerase during this process. The structure of an empty mumps virus (MuV) nucleocapsid-like particle was determined to 10.4-Å resolution by cryo-electron microscopy (cryo-EM) image reconstruction. By modeling the crystal structure of parainfluenza virus 5 into the density, it was shown that the α-helix close to the RNA became flexible when RNA was removed. Point mutations in this helix resulted in loss ..View full abstract
Awarded by NIGMS NIH HHS
Awarded by NIAID NIH HHS
Awarded by NICHD NIH HHS