Journal article

HIV integration sites in latently infected cell lines: evidence of ongoing replication

Jori Symons, Abha Chopra, Eva Malantinkova, Ward De Spiegelaere, Shay Leary, Don Cooper, Chike O Abana, Ajantha Rhodes, Simin D Rezaei, Linos Vandekerckhove, Simon Mallal, Sharon R Lewin, Paul U Cameron

RETROVIROLOGY | BIOMED CENTRAL LTD | Published : 2017

Abstract

BACKGROUND: Assessing the location and frequency of HIV integration sites in latently infected cells can potentially inform our understanding of how HIV persists during combination antiretroviral therapy. We developed a novel high throughput sequencing method to evaluate HIV integration sites in latently infected cell lines to determine whether there was virus replication or clonal expansion in these cell lines observed as multiple integration events at the same position. RESULTS: We modified a previously reported method using random DNA shearing and PCR to allow for high throughput robotic processing to identify the site and frequency of HIV integration in latently infected cell lines. Late..

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Grants

Awarded by National Health and Medical Research Council, NHMRC


Awarded by Delaney AIDS Research Enterprise, DARE


Awarded by Agency for Innovation by Science and Technology of the Flemish Government, IWT


Awarded by Research Foundation-Flanders, FWO


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

PUC; National Health and Medical Research Council, NHMRC, APP1058891. SRL; Delaney AIDS Research Enterprise, DARE, U19 AI96109-01, National Health and Medical Research Council, NHMRC APP1052979; 1058891. EM; Agency for Innovation by Science and Technology of the Flemish Government, IWT, 111286. LV; Research Foundation-Flanders, FWO, 1.8.020.09. N.00.WDS; Research Foundation-Flanders, FWO, 12G9716N.