Evidence of Platelet Activation at Medically Used Hypothermia and Mechanistic Data Indicating ADP as a Key Mediator and Therapeutic Target
Andreas Straub, Stefanie Krajewski, Jan David Hohmann, Erik Westein, Fu Jia, Nicole Bassler, Carly Selan, Julia Kurz, Hans Peter Wendel, Shala Dezfouli, Yuping Yuan, Harshal Nandurkar, Shaun Jackson, Michael J Hickey, Karlheinz Peter
Arteriosclerosis Thrombosis and Vascular Biology | LIPPINCOTT WILLIAMS & WILKINS | Published : 2011
OBJECTIVE: Hypothermia is used in various clinical settings to inhibit ischemia-related organ damage. However, prothrombotic effects have been described as potential side effects. This study aimed to elucidate the mechanism of hypothermia-induced platelet activation and subsequent prothrombotic events and to develop preventative pharmacological strategies applicable during clinically used hypothermia. METHODS AND RESULTS: Platelet function was investigated ex vivo and in vivo at clinically used hypothermia (28°C/18°C). Hypothermic mice demonstrated increased expression of platelet activation marker P-selectin, platelet-leukocyte aggregate formation, and thrombocytopenia. Intravital microscop..View full abstract
Awarded by German Research Foundation
Dr Straub was supported by a grant from the German Research Foundation (STR 687/1-1); Dr Peter was supported by the Australian Research Council; and Drs Bassler, Jackson, Hickey, and Peter were supported by the National Health and Medical Research Council of Australia.