13 Gene Expression Pattern of Arabidopsis EXPB1, a Nonallergenic Homologue of Grass Group 1 Pollen Allergens

Abstract

Background Grass pollen allergy is one of the most common allergies worldwide. Group I allergens constitute the major allergenic component of grass pollen with more than 85% of grass pollen allergic patients showing IgE reactivity. These are highly immunologically cross-reactive glycoproteins specifically expressed in pollen of all grasses. Alignments of the amino-acid sequences of grass group I allergens derived from diverse grass species reveal up to 95% homology. It is therefore likely that these molecules share a similar biological function. Methods RT-PCR analysis, In situ hybridisation, Promoter-GFP construct design, plant transformation and analysis of transgenic plants. Results Seque..