Infection Programs Sustained Lymphoid Stromal Cell Responses and Shapes Lymph Node Remodeling upon Secondary Challenge

JL Gregory; A Walter; YO Alexandre; JL Hor; R Liu; JZ Ma; S Devi; N Tokuda; Y Owada; LK Mackay; GK Smyth; WR Heath; SN Mueller

Journal article

Infection Programs Sustained Lymphoid Stromal Cell Responses and Shapes Lymph Node Remodeling upon Secondary Challenge

JL Gregory, A Walter, YO Alexandre, JL Hor, R Liu, JZ Ma, S Devi, N Tokuda, Y Owada, LK Mackay, GK Smyth, WR Heath, SN Mueller

Cell Reports | CELL PRESS | Published : 2017

DOI: 10.1016/j.celrep.2016.12.038

Abstract

Lymph nodes (LNs) are constructed of intricate networks of endothelial and mesenchymal stromal cells. How these lymphoid stromal cells (LSCs) regulate lymphoid tissue remodeling and contribute to immune responses remains poorly understood. We performed a comprehensive functional and transcriptional analysis of LSC responses to skin viral infection and found that LSC subsets responded robustly, with different kinetics for distinct pathogens. Recruitment of cells to inflamed LNs induced LSC expansion, while B cells sustained stromal responses in an antigen-independent manner. Infection induced rapid transcriptional responses in LSCs. This transcriptional program was transient, returning to hom..

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University of Melbourne Researchers

Yannick Alexandre Author

Sapna Devi Author

Laura Mackay Author

Gordon Smyth Author

Related Projects (1)

Defining the immunological roles of stromal cells within lymphoid tissues

The populations of endothelial and mesenchymal cells that construct the lymphoid tissues are being revealed as key players in the priming an..

Grants

Awarded by National Institutes of Health

Funding Acknowledgements

We thank Rafi Ahmed (Emory University, GA) for LCMV virus, Weisan Chen (La Trobe University) for vaccinia virus (which was originally a gift from Jonathan Yewdell and Jack Bennink [NIH, Bethesda, MD]), Michael Hickey (Monash University) for PS/2 and M17/4 antibodies, and Hidde Ploegh (Whitehead Institute, Cambridge, MA) for OBI mice. This work was supported by the Australian Research Council (DP140101246).