Journal article
CCL2-driven inflammation increases mammary gland stromal density and cancer susceptibility in a transgenic mouse model
X Sun, DJ Glynn, LJ Hodson, C Huo, K Britt, EW Thompson, L Woolford, A Evdokiou, JW Pollard, SA Robertson, WV Ingman
Breast Cancer Research | BMC | Published : 2017
Open access
Abstract
Background: Macrophages play diverse roles in mammary gland development and breast cancer. CC-chemokine ligand 2 (CCL2) is an inflammatory cytokine that recruits macrophages to sites of injury. Although CCL2 has been detected in human and mouse mammary epithelium, its role in regulating mammary gland development and cancer risk has not been explored. Methods: Transgenic mice were generated wherein CCL2 is driven by the mammary epithelial cell-specific mouse mammary tumour virus 206 (MMTV) promoter. Estrous cycles were tracked in adult transgenic and non-transgenic FVB mice, and mammary glands collected at the four different stages of the cycle. Dissected mammary glands were assessed for cycl..
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Awarded by National Breast Cancer Foundation
Funding Acknowledgements
This work was supported by the Australian National Breast Cancer Foundation (NBCF), the Hospital Research Foundation (THRF), the Victorian Breast Cancer Research Consortium, the St. Vincent's Hospital Research Endowment Fund, and the University of Melbourne Research Grant Support Scheme, the Wellcome Trust 101067/Z/13/Z (JWP), Medical Research Council Centre for Reproductive Health grant G1002033 (JWP) and NIH grants RO1 CA172451 (JWP). XS was supported by University of Adelaide Graduate Research Scholarship and Florey Medical Research Foundation Postgraduate Cancer Research Top Up Scholarship. CWH was supported by the Australian Postgraduate Awards scholarship. KB is supported by an NBCF Early Career Fellowship. EWT was supported in part by the NBCF. AE is a THRF Fellow. WVI is an NBCF/THRF Fellow.