Journal article

GENOMIC ORGANIZATION OF THE MOUSE PORE-FORMING PROTEIN (PERFORIN) GENE AND LOCALIZATION TO CHROMOSOME-10 - SIMILARITIES TO AND DIFFERENCES FROM C9

JA TRAPANI, BS KWON, CA KOZAK, C CHINTAMANENI, JDE YOUNG, B DUPONT

Journal of Experimental Medicine | ROCKEFELLER UNIV PRESS | Published : 1990

Abstract

Genomic clones encompassing the entire coding region of the mouse lymphocyte pore-forming protein gene (Pfp) have been isolated and used to determine its intron-exon organization. In contrast to C9, Pfp has a simple structure, consisting of only three exons (two of which encode polypeptide), a large 5' intron, and a single, smaller intron that is situated approximately one-third of the way through the protein-coding portions of the gene. The regions encoding the homologous domains of PFP and C9 are encoded on exons 7, 8, 9, and 10 of C9, but form only approximately half of the open reading frame of exon III in Pfp. Although encoding polypeptides with related functions, the two genes possess ..

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