Journal article

ISOLATION AND CHARACTERIZATION OF CDNA CLONES FOR MOUSE LY-9

MS SANDRIN, TP GUMLEY, MM HENNING, HA VAUGHAN, LJ GONEZ, JA TRAPANI, IFC MCKENZIE

Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 1992

Abstract

We describe the production and characterization of a mouse mAb, S-450-33.2, recognizing the Ly-9.2 specificity. This mAb was used to purify Ly-9 molecules from lymphoid cell lines, and the amino-terminal amino acids were determined. The mAb was also used in a eukaryotic expression system, to isolate cDNA clones encoding Ly-9. Analysis of RNA showed that Ly-9 expression is lymphocyte specific, as determined by the presence of a single hybridizing 2.4-kb species found only in lymphoid cells. Genomic DNA analysis indicated that Ly-9 is encoded by a single-copy gene of 10 to 15 kb. The predicted polypeptide belongs to the Ig superfamily of cell surface molecules with four extracellular Ig-like d..

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