Journal article

Interleukin-1 beta-converting enzyme-like protease cleaves DNA-dependent protein kinase in cytotoxic T cell killing

QZ Song, SR Burrows, G Smith, SP LeesMiller, S Kumar, DW Chan, JA Trapani, E Alnemri, G Litwack, H Lu, DJ Moss, S Jackson, MF Lavin

The Journal of Experimental Medicine | ROCKEFELLER UNIV PRESS | Published : 1996

Abstract

Cytotoxic T cells (CTL) represent the major defense mechanism against the spread of virus infection. It is believed that the pore-forming protein, perforin, facilitates the entry of a series of serine proteases (particularly granzyme B) into the target cell which ultimately leads to DNA fragmentation and apoptosis. We demonstrate here that during CTL-mediated cytolysis the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), an enzyme implicated in the repair of double strand breaks in DNA, is specifically cleaved by an interleukin (IL)-1 beta-converting enzyme (ICE)-like protease. A serine protease inhibitor, 3,4-dichloroisocoumarin (DCl), which is known to block granzyme B activit..

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