Journal article

Expression of recombinant human Met-ase-1: A NK cell-specific granzyme

MJ Smyth, MD OConnor, JM Kelly, P Ganesvaran, KYT Thia, JA Trapani

Biochemical and Biophysical Research Communications | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Published : 1995

Abstract

Human Met-ase-1 is a member of a family of cytotoxic lymphocyte serine proteases (granzymes), but is expressed specifically in CD3- large granular lymphocytes with natural killer cell activity. We have devised a polymerase chain reaction strategy to delete the predicted hexapropeptide of human Met-ase-1 (Ser-6 to Gln-1), to enable its expression and activation in mammalian COS cells. In addition, using peptide immunization we have derived a unique and specific monoclonal antibody detecting human Met-ase-1. Western blot analysis and protease assays of transfected COS cell lysates against a panel of thiobenzyl ester substrates formally demonstrated that the human Met-ase-1 gene encodes a serin..

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