Journal article

MET-ASE - CLONING AND DISTINCT CHROMOSOMAL LOCATION OF A SERINE-PROTEASE PREFERENTIALLY EXPRESSED IN HUMAN NATURAL-KILLER-CELLS

MJ SMYTH, TJ SAYERS, T WILTROUT, JC POWERS, JA TRAPANI

Journal of Immunology | AMER ASSOC IMMUNOLOGISTS | Published : 1993

Abstract

A cDNA clone encoding a human NK serine protease was obtained by screening a lambda-gt10 library from the Lopez NK leukemia with the rat natural killer Met-ase (RNK-Met-1) cDNA clone. In Northern blot analysis human Met-ase (Hu-Met-1) cDNA hybridized with a 0.9-kb mRNA in two human NK leukemia cell lines, unstimulated human PBMC, and untreated purified CD3-CD56+ large granular lymphocytes. Unlike other members of the granzyme family that are highly expressed in activated peripheral T cells, the Hu-Met-1 transcript was barely detected in a population of PMA and ionomycin or IL-2-treated high density T cells. Several in vitro cultured Burkitt lymphomas, chronic- and promyeloid leukemias, acute..

View full abstract