Journal article

mRNA Export from Mammalian Cell Nuclei Is Dependent on GANP

Vihandha O Wickramasinghe, Paul IA McMurtrie, Anthony D Mills, Yoshinori Takei, Sue Penrhyn-Lowe, Yoko Amagase, Sarah Main, Jackie Marr, Murray Stewart, Ronald A Laskey



Bulk nuclear export of messenger ribonucleoproteins (mRNPs) through nuclear pore complexes (NPCs) is mediated by NXF1. It binds mRNPs through adaptor proteins such as ALY and SR splicing factors and mediates translocation through the central NPC transport channel via transient interactions with FG nucleoporins. Here, we show that mammalian cells require GANP (germinal center-associated nuclear protein) for efficient mRNP nuclear export and for efficient recruitment of NXF1 to NPCs. Separate regions of GANP show local homology to FG nucleoporins, the yeast mRNA export factor Sac3p, and the mammalian MCM3 acetyltransferase. GANP interacts with both NXF1 and NPCs and partitions between NPCs and..

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Awarded by Medical Research Council

Awarded by MRC

Funding Acknowledgements

This study was funded by the Medical Research Council and Cancer Research UK. P.I.A.M. was supported by an MB/PhD studentship funded by Merck Sharp & Dohme. M.S. is supported by a programme grant from the Wellcome Trust.