Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export
Divyang Jani, Sheila Lutz, Ed Hurt, Ronald A Laskey, Murray Stewart, Vihandha O Wickramasinghe
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2012
Export of messenger RNA (mRNA) from the nucleus to the cytoplasm is a critical step in the gene expression pathway of eukaryotic cells. Here, we report the functional and structural characterization of the mammalian TREX-2 complex and show how it links transcription/processing with nuclear mRNA export. Mammalian TREX-2 is based on a germinal-centre associated nuclear protein (GANP) scaffold to which ENY2, PCID2 and centrins bind and depletion of any of these components inhibits mRNA export. The crystal structure of the GANP:ENY2 complex shows that two ENY2 chains interact directly with GANP, but they have different orientations from those observed on yeast Sac3. GANP is required to recruit E..View full abstract
Awarded by Medical Research Council (MRC)
Awarded by Medical Research Council
V.O.W. acknowledges the support of Ashok Venkitaraman during the latter half of this study. S. L. was a recipient of an EMBO short-term fellowship to study in the lab of RAL.Medical Research Council (MRC) and Cancer Research UK; Wellcome Trust and Medical Research Council grant (U105178939 to M. S.) in part. Funding for open access charge: University of Cambridge, Medical Research Council, Wellcome Trust.