Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP
Vihandha O Wickramasinghe, Robert Andrews, Peter Ellis, Cordelia Langford, John B Gurdon, Murray Stewart, Ashok R Venkitaraman, Ronald A Laskey
NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2014
The nuclear phase of the gene expression pathway culminates in the export of mature messenger RNAs (mRNAs) to the cytoplasm through nuclear pore complexes. GANP (germinal- centre associated nuclear protein) promotes the transfer of mRNAs bound to the transport factor NXF1 to nuclear pore complexes. Here, we demonstrate that GANP, subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent tran..View full abstract
Awarded by MRC
Awarded by Medical Research Council
MRC (to R. A. L., V.O.W., A. R. V. and M. S.); MRC Programme grant [G1001522 to A. R. V. supports V.O.W.]; MRC Programme grant [U105178939 to M. S.]; CRUK (to R. A. L., V.O.W. and M. S.); Wellcome Trust (to C. L., P. E., R. A. and M. S.). Funding for open access charge: University of Cambridge.