Journal article
Microtubule-independent secretion requires functional maturation of Golgi elements
L Fourriere, S Divoux, M Roceri, F Perez, G Boncompain
Journal of Cell Science | COMPANY OF BIOLOGISTS LTD | Published : 2016
DOI: 10.1242/jcs.188870
Abstract
The Golgi complex is responsible for processing and sorting of secretory cargos. Microtubules are known to accelerate the transport of proteins from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi to the plasma membrane. However, whether post- Golgi transport strictly requires microtubules is still unclear. Using the retention using selective hooks (RUSH) system to synchronize the trafficking of cargos, we show that anterograde transport of tumor necrosis factor (TNF) is strongly reduced without microtubules. We show that two populations of Golgi elements co-exist in these cells. A centrally located and giantin-positive Golgi complex that sustains trafficking, and newl..
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Awarded by Agence Nationale de la Recherche
Funding Acknowledgements
Electron microscopy work was supported by the Agence Nationale de la Recherche through the 'Investments for the Future' program (France-BioImaging) [grant number ANR-10-INSB-04]. We acknowledge the BioImaging Cell and Tissue Core Facility of the Institut Curie (PICT-IBiSA), a member of the France-BioImaging national research infrastructure, supported by the CelTisPhyBio Labex [grant number ANR-10-LBX-0038] part of the IDEX PSL [grant number ANR-10-IDEX-0001-02 PSL]. The work in the laboratory of F.P. was supported by the Institut Curie, the Centre National de la Recherche Scientifique (CNRS) and by grants from the Fondation pour la Recherche Medicale [grant numbers DEQ20120323723 and FDT20150532154] and from the Agence Nationale de la Recherche [grant number ANR-12-BSV2-0003-01].