Journal article
SCA-1 Labels a Subset of Estrogen-Responsive Bipotential Repopulating Cells within the CD24 CD49fhi Mammary Stem Cell-Enriched Compartment
GV Dall, JL Vieusseux, KS Korach, Y Arao, SC Hewitt, KJ Hamilton, E Dzierzak, WC Boon, ER Simpson, RG Ramsay, T Stein, JS Morris, RL Anderson, GP Risbridger, KL Britt
Stem Cell Reports | CELL PRESS | Published : 2017
Abstract
Estrogen stimulates breast development during puberty and mammary tumors in adulthood through estrogen receptor-α (ERα). These effects are proposed to occur via ERα+ luminal cells and not the mammary stem cells (MaSCs) that are ERαneg. Since ERα+ luminal cells express stem cell antigen-1 (SCA-1), we sought to determine if SCA-1 could define an ERα+ subset of EpCAM+/CD24+/CD49fhi MaSCs. We show that the MaSC population has a distinct SCA-1+ population that is abundant in pre-pubertal mammary glands. The SCA-1+ MaSCs have less stem cell markers and less in vivo repopulating activity than their SCA-1neg counterparts. However, they express ERα and specifically enter the cell cycle at puberty. Us..
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Awarded by Division of Intramural Research, National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
G.D., Australian Postgradutate Scholarship; K.B., NBCF ECR Fellowship (ECF 11-01), NHMRC New Investigator grant (APP1044661), and VCA ECR fellowship (ECSG08_07); R.L.A., NBCF Senior Fellowship; K.S. K., Division of Intramural Research/NIEHS [1ZIAESO70065]; G.P.R., NHMRC fellowship. We thank Dr. Carl Walkey (St Vincents Institute) and A/Prof Steve Lane (QIMR Berghofer) for their helpful discussions about cell-cycle-specific staining, the flow core facility at Monash University, and the FACS facility at Peter Mac as well as Monash Micro Imaging Facility and Peter Mac microscopy groups for provision of instrumentation, training, and general support.