Rapid purification of the gastric H /K -ATPase complex by tomato-lectin affinity chromatography

Abstract

We have previously shown that tomato lectin binds specifically to the 60-90 kDa membrane glycoprotein of parietal cell tubulovesicles, the β-subunit of the gastric H+/K+-ATPase (proton pump) [Callaghan, Toh, Pettitt, Humphris and Gleeson (1990) J. Cell Sci. 95, 563-576; Toh, Gleeson, Simpson, Moritz, Callaghan, Goldkorn, Jones, Martinelli, Mu, Humphris, Pettitt, Mori, Masuda, Sobieszczuk, Weinstock, Mantamadiotis and Baldwin (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 6418-6422]. Here we have exploited this interaction for the development of a rapid single-step chromatography procedure for the purification of an active pig gastric proton pump complex. Initially H+/K+-ATPase-enriched membranes p..