Galactosyl transferases of baby hamster kidney (BHK) cells: Characterization of two oligosaccharide products synthesised using bovine asialo submaxillary‐gland mucin as acceptor

Abstract

Extracts of BHK (baby hamster kidney) cells catalyse incorporation of galactose from UDP‐galactose into asialo bovine submaxillary gland mucin. The galactosylated oligosaccharide products were released by alkaline‐borohydride treatment and purified by Bio‐Gel P2 chromatography and high‐performance liquid chromatography. The structures of the oligosaccharide sequences synthesised have been identified unequivocally by high resolution 500 MHz 1H‐NMR as galactosyl‐(β→3) N‐acetylgalactosamine and galactosyl (β→4) N‐acetylglucosaminyl (β→3)‐N‐acetylgalactosamine. Characterization of the latter sequence shows the presence in bovine mucin of the type III core sequence N‐acetylglucosamine‐(β→3) N‐ace..