Journal article

GALACTOSYL TRANSFERASES OF BABY HAMSTER-KIDNEY (BHK) CELLS - CHARACTERIZATION OF 2 OLIGOSACCHARIDE PRODUCTS SYNTHESIZED USING BOVINE ASIALO SUBMAXILLARY-GLAND MUCIN AS ACCEPTOR

PA GLEESON, J FEENEY, G MILLS, RC HUGHES

The FEBS Journal | SPRINGER VERLAG | Published : 1984

Abstract

Extracts of BHK (baby hamster kidney) cells catalyse incorporation of galactose from UDP-galactose into asialo bovine submaxillary gland mucin. The galactosylated oligosaccharide products were released by alkaline-borohydride treatment and purified by Bio-Gel P2 chromatography and high-performance liquid chromatography. The structures of the oligosaccharide sequences synthesised have been identified unequivocally by high resolution 500 MHz 1H-NMR as galactosyl-(beta 1----3) N-acetylgalactosamine and galactosyl (beta 1----4) N-acetylglucosaminyl (beta 1----3)-N-acetylgalactosamine. Characterization of the latter sequence shows the presence in bovine mucin of the type III core sequence N-acety..

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