Journal article

p230 is associated with vesicles budding from the trans-Golgi network

PA Gleeson, TJ Anderson, JL Stow, G Griffiths, BH Toh, F Matheson

Journal of Cell Science | COMPANY OF BIOLOGISTS LTD | Published : 1996


Transport vesicle formation requires the association of cytosolic proteins with the membrane. We have previously described a brefeldin-A sensitive, hydrophilic protein (p230), containing a very high frequency of heptad repeats, found in the cytosol and associated with Golgi membranes. We show here that p230 is localised on the trans-Golgi network, by immunogold labeling of HeLa cell cryosections using alpha 2,6 sialyltransferase as a compartment-specific marker. The role of G protein activators on the binding of p230 to Golgi membranes and in vesicle biogenesis has been investigated. Treatment of streptolysin-O permeabilised HeLa cells with either GTP gamma S or AlF4- resulted in accumulatio..

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