Heterogeneity of human anti‐Golgi auto‐antibodies: Reactivity with components from 35 to 260 kDa

Abstract

The Golgi auto‐antigens recognized by five human autoimmune sera were characterized with anti‐Golgi auto‐antibodies. The five sera showed strong anti‐Golgi reactivity, together with weak anti‐nuclear reactivity, as assessed by indirect immunofluorescence using the human HEp2 epithelial cell line. The Golgi auto‐antigens recognized by the autoimmune sera were identified by immunoprecipitation and immunoblotting of post‐nuclear supernatants. These sera reacted with at least 14 components of molecular mass (Mr) 35–260 kDa; three components were detected only by immunoprecipitation, six components detected only by immunoblotting and five components appear to be detected by both techniques. We ha..