Journal article

POLY-N-ACETYLLACTOSAMINE-SPECIFIC TOMATO LECTIN INTERACTS WITH GASTRIC PARIETAL-CELLS - IDENTIFICATION OF A TOMATO-LECTIN BINDING 60-90X103MR MEMBRANE GLYCOPROTEIN OF TUBULOVESICLES

JM CALLAGHAN, BH TOH, JM PETTITT, DC HUMPHRIS, PA GLEESON

Journal of Cell Science | COMPANY OF BIOLOGISTS LTD | Published : 1990

Abstract

The cytoplasmic tubulovesicular and canalicular membranes of gastric parietal cells are intimately involved in hydrochloric acid secretion. To characterise the glycoproteins of these membranes, we examined a panel of lectins for reactivity with parietal cells in paraffin sections of rat, dog and pig stomach. The poly-N-acetyllactosamine-specific lectin from Lycopersicon esculentum (tomato) and from Solanum tuberosum (potato), and the galactose-specific lectin Ricinus communis agglutinin (RCA120), showed strong cytoplasmic binding of parietal cells of all three species, with a pattern indicative of an intracellular membrane network. Binding to parietal cells was confirmed by double-labelling ..

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