Journal article

BINDING AND UPTAKE OF THE TOXIC LECTIN MODECCIN BY BABY HAMSTER-KIDNEY (BHK) CELLS - ISOLATION OF MUTANTS DEFECTIVE IN THE INTERNALIZATION OF MODECCIN

PA GLEESON, RC HUGHES

JOURNAL OF CELL SCIENCE | COMPANY OF BIOLOGISTS LTD | Published : 1985

Abstract

The effects of the toxic lectins, ricin and modeccin, on baby hamster kidney (BHK) cells have been compared. Modeccin is about 20-50 times more toxic to BHK cells than ricin. Binding studies showed that there are 10(5) to 2 X 10(5) modeccin binding sites/cell compared with approximately 10(7) binding sites for ricin. Inhibition studies with galactosides indicate that both N- and O-glycans with terminal galactosyl residues are effective inhibitors of modeccin binding. Surface-bound modeccin, at 0 degrees C, was found to be very rapidly endocytosed (75-80% in 10 min) when cells were warmed to 37 degrees C. Two modeccin-resistant BHK cell lines have been isolated and characterized. These mutant..

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