Journal article

HUMAN AUTOANTIBODIES AS REAGENTS TO CONSERVED GOLGI COMPONENTS - CHARACTERIZATION OF A PERIPHERAL, 230-KDA COMPARTMENT-SPECIFIC GOLGI PROTEIN

J KOOY, BH TOH, JM PETTITT, R ERLICH, PA GLEESON

Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 1992

Abstract

We have used a serum from a patient with Sjögren's syndrome containing high titer (100,000) anti-Golgi autoantibodies and lower titer (20,000) anti-nuclear autoantibodies to characterize the Golgi complex. The Sjögren's syndrome serum immunoprecipitated a number of components of molecular mass 35-230 kDa from detergent extracts of [35S]methionine-labeled HeLa cells; at high dilution, the serum precipitated one major 230-kDa component. Using the Sjögren's syndrome serum, cDNA clones encoding the Golgi autoantigen were isolated from a lambda gt11 HeLa cell cDNA library. Autoantibodies from the Sjögren's syndrome serum, affinity purified from a recombinant bacterial fusion protein generated fro..

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