Identification of components of the endoplasmic reticulum and Golgi complex by murine autoreactive monoclonal antibodies

Abstract

A number of autoreactive monoclonal antibodies have been produced by the fusion of spleen cells from unprimed BALB/c mice. The specificities of two of these monoclonal autoantibodies, MUI 38 and MUI 100, have been further examined. By indirect immunofluorescence, monoclonal antibody MUI 38 showed discrete perinuclear staining of acetone-fixed murine 3T3 fibroblasts, which was similar to that obtained with the Golgi vital stain, C6-NBD-ceramide, and with rhodamine-labelled wheat germ agglutinin. Furthermore, the staining pattern with antibody MUI 38 in cells treated with either monensin, taxol or nocodazol was altered in a manner consistent with the known effects of these drugs on Golgi morph..