Journal article

N-LINKED OLIGOSACCHARIDES OF THE MURINE TRANSFERRIN RECEPTOR FROM A PLASMACYTOMA CELL-LINE - COMPARISON WITH TOTAL CELLULAR N-GLYCANS

PA GLEESON, V DIAS

BIOCHIMICA ET BIOPHYSICA ACTA | ELSEVIER SCIENCE BV | Published : 1990

Abstract

The N-linked oligosaccharides synthesised by the murine plasmacytoma cell line NS-1 have been analysed by lectin affinity chromatography on columns of immobilised concanavalin A (Con A), Lens culinaris (lentil), Ricinus communis agglutinin (RCA) and leuko-phytohemagglutinin (L-PHA). The majority of complex N-glycans in this transformed cell line were branched structures with only a low level of biantennary complex chains detected. The analysis showed the major complex N-glycan fraction consisted of a minimum sialylated triantennary structure. [3H]Mannose-labelled transferrin receptor was isolated from NS-1 cells by immunoprecipitation followed by electroelution from SDS polyacrylamide gels. ..

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