Journal article

THE TRANSMEMBRANE AND FLANKING SEQUENCES OF BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE-I SPECIFY MEDIAL-GOLGI LOCALIZATION

J BURKE, JM PETTITT, H SCHACHTER, M SARKAR, PA GLEESON

Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 1992

Abstract

UDP-GlcNAc:alpha 3-D-mannoside beta 1,2-N-acetylglucosaminyltransferase I (GnTI) is an N(in)/C(out) (type II) membrane protein, localized in the medial-Golgi, that initiates the conversion of high mannose N-glycans to complex N-glycans. Anti-rabbit GnTI antibodies were generated using a purified, enzymatically active, bacterial recombinant fusion protein as immunogen. Rabbit GnTI was effectively retained in the Golgi complex of transfected COS-1 cells and murine L cells, as assessed by indirect immunofluorescence using the species-specific anti-GnTI antibodies; no surface expression of rabbit GnTI could be detected in the transfected cells. Rabbit GnTI, stably expressed in murine L cells, wa..

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