Journal article
Three Distinct Patterns of Histone H3Y41 Phosphorylation Mark Active Genes
MA Dawson, SD Foster, AJ Bannister, SC Robson, R Hannah, X Wang, B Xhemalce, AD Wood, AR Green, B Göttgens, T Kouzarides
Cell Reports | CELL PRESS | Published : 2012
Abstract
The JAK2 tyrosine kinase is a critical mediator of cytokine-induced signaling. It plays a role in the nucleus, where it regulates transcription by phosphorylating histone H3 at tyrosine 41 (H3Y41ph). We used chromatin immunoprecipitation coupled to massively parallel DNA sequencing (ChIP-seq) to define the genome-wide pattern of H3Y41ph in human erythroid leukemia cells. Our results indicate that H3Y41ph is located at three distinct sites: (1) at a subset of active promoters, where it overlaps with H3K4me3, (2) at distal cis-regulatory elements, where it coincides with the binding of STAT5, and (3) throughout the transcribed regions of active, tissue-specific hematopoietic genes. Together, t..
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Awarded by Wellcome Trust
Funding Acknowledgements
We would like to thank Yongjun Zhao for overseeing the sequencing and Sarah Teichmann for helpful discussions. Work in the authors' laboratories is supported by grants from Cancer Research UK, Leukaemia & Lymphoma Research UK, the UK Medical Research Council, the Wellcome Trust, Leukemia & Lymphoma Society of America, NIHR Cambridge Biomedical Research Centre, and the 6th Research Framework Programme of the European Union (Epitron and SMARTER). M. A. D. is a Wellcome-Beit Intermediate Clinical Fellow. S. D. F. was funded by a PhD fellowship grant from the UK Medical Research Council to the Cambridge Institute for Medical Research. T. K. is a director of Abcam Ltd and he is on the scientific advisory board of GlaxoSmithKline. A. R. G. is on the clinical advisory board of Astex Therapeutics, Cambridge, UK.