Journal article
A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging
CL Fleming, TD Ashton, C Nowell, M Devlin, A Natoli, J Schreuders, FM Pfeffer
Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2015
DOI: 10.1039/c5cc02059j
Open access
Abstract
Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify 'profluorogenic' substrates for intracellular studies.
Grants
Awarded by Australian Research Council
Funding Acknowledgements
CLF thanks the Research Centre for Chemistry and Biotechnology for a top-up scholarship. The authors would like to acknowledge the Australian Research Council for funding Deakin University's Nuclear Magnetic Resonance Facility through LIEF grant LE110100141, as well as for additional equipment support related to this project (LE120100213).