Journal article

Comparison of multimeric plus and minus forms of viroids and virusoids.

CJ Hutchins, P Keese, JE Visvader, PD Rathjen, JL McInnes, RH Symons

Plant Molecular Biology | Published : 1985

Abstract

In order to investigate the mechanism of replication of viroids and virusoids, we have compared the replication intermediates of three members of each group in nucleic acid extracts of infected plants. Viroids were avocado sunblotch viroid (ASBV), citrus exocortis viroid (CEV) and coconut cadang cadang viroid (CCCV). Virusoids were from velvet tobacco mottle virus (VTMoV), solanum nodiflorum mottle virus (SNMV) and lucerne transient streak virus (LTSV). Analysis of intermediates was by the Northern hybridization technique with single-strand DNA and RNA probes prepared from recombinant DNA clones. The results obtained are discussed in terms of current models of viroid and virusoid replication..

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University of Melbourne Researchers

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