Journal article
Quaternary structure of WzzB and WzzE polysaccharide copolymerases
S Kalynych, M Cherney, M Bostina, I Rouiller, M Cygler
Protein Science | WILEY-BLACKWELL | Published : 2015
DOI: 10.1002/pro.2586
Abstract
Bacteria have evolved cellular control mechanisms to ensure proper length specification for surface-bound polysaccharides. Members of the Polysaccharide Copolymerase (PCP) family are central to this process. PCP-1 family members are anchored to the inner membrane through two transmembrane helices and contain a large periplasm-exposed domain. PCPs are known to form homooligomers but their exact stoichiometry is controversial in view of conflicting structural and biochemical data. Several prior investigations addressing this question indicated a nonameric, hexameric, or tetrameric organization of several PCP-1 family members. In this work, we gathered additional evidence that E.coli WzzB and W..
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Funding Acknowledgements
The research described in this paper was performed in part using CMCF beamline 08ID-1 at the Canadian Light Source, which is supported by the Natural Sciences and Engineering Research Council of Canada, the National Research Council Canada, the Canadian Institutes of Health Research, the Province of Saskatchewan, Western Economic Diversification Canada, and the University of Saskatchewan, and at the GM/CA-CAT beamline ID23 at the Advanced Photon Source, Argonne National Laboratory. The EM was performed at the Facility for Electron Microscopy Research at McGill University. They would like to thank Shaun Labiuk, Michel Fodje, Pawel Grochulski, Deqiang Yao, and Craig Ogata for copious personal help with crystal screening.