Long-Term Nickel Contamination Increases the Occurrence of Antibiotic Resistance Genes in Agricultural Soils
Hang-Wei Hu, Jun-Tao Wang, Jing Li, Xiu-Zhen Shi, Yi-Bing Ma, Deli Chen, Ji-Zheng He
ENVIRONMENTAL SCIENCE & TECHNOLOGY | AMER CHEMICAL SOC | Published : 2017
Heavy metal contamination is assumed to be a selection pressure on antibiotic resistance, but to our knowledge, evidence of the heavy metal-induced changes of antibiotic resistance is lacking on a long-term basis. Using quantitative PCR array and Illumina sequencing, we investigated the changes of a wide spectrum of soil antibiotic resistance genes (ARGs) following 4-5 year nickel exposure (0-800 mg kg-1) in two long-term experimental sites. A total of 149 unique ARGs were detected, with multidrug and β-lactam resistance as the most prevailing ARG types. The frequencies and abundance of ARGs tended to increase along the gradient of increasing nickel concentrations, with the highest values re..View full abstract
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Awarded by Chinese Academy of Sciences
Awarded by Australian Research Council
This work was financially supported by the Chinese Academy of Sciences (XDB15020200), and the Australian Research Council (DE150100870, DP170103628).