A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model
CS McLachlan, JL Yin, C Driussi, PR Jusuf, B Hambly, MA McGuire
The Journal of Physiological Sciences | CENTER ACADEMIC PUBL JAPAN | Published : 2000
Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.