Journal article

Monoclonal Antibodies Directed toward the Hepatitis C Virus Glycoprotein E2 Detect Antigenic Differences Modulated by the N-Terminal Hypervariable Region 1 (HVR1), HVR2, and Intergenotypic Variable Region

Yousef Alhammad, Jun Gu, Irene Boo, David Harrison, Kathleen McCaffrey, Patricia T Vietheer, Stirling Edwards, Charles Quinn, Fasseli Coulibaly, Pantelis Poumbourios, Heidi E Drummer

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2015

Abstract

UNLABELLED: Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 form a heterodimer and mediate receptor interactions and viral fusion. Both E1 and E2 are targets of the neutralizing antibody (NAb) response and are candidates for the production of vaccines that generate humoral immunity. Previous studies demonstrated that N-terminal hypervariable region 1 (HVR1) can modulate the neutralization potential of monoclonal antibodies (MAbs), but no information is available on the influence of HVR2 or the intergenotypic variable region (igVR) on antigenicity. In this study, we examined how the variable regions influence the antigenicity of the receptor binding domain of E2 spanning HCV polyprot..

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University of Melbourne Researchers

Grants

Awarded by NHMRC


Awarded by NHMRC senior research fellowship


Awarded by R. D. Wright fellowship


Funding Acknowledgements

This work was supported by NHMRC project grants 1020175, 543113, and 1009809 and the Australian Centre for HIV and Hepatitis Virology. H.E.D. is currently supported by NHMRC senior research fellowship 1041897 and was previously supported by R. D. Wright fellowship 433929. F.C. is a Future Fellow of the Australian Research Council. We gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program, received by the Burnet Institute.