Journal article
A genome-wide screen to identify transcription factors expressed in pelvic ganglia of the lower urinary tract
CB Wiese, S Ireland, NL Fleming, J Yu, MT Valerius, K Georgas, HS Chiu, J Brennan, J Armstrong, MH Little, AP McMahon, EM Southard-Smith
Frontiers in Neuroscience | FRONTIERS MEDIA SA | Published : 2012
Abstract
Relative positions of neurons within mature murine pelvic ganglia based on expression of neurotransmitters have been described. However the spatial organization of developing innervation in the murine urogenital tract (UGT) and the gene networks that regulate specification and maturation of neurons within the pelvic ganglia of the lower urinary tract (LUT) are unknown.We used whole-mount immunohistochemistry and histochemical stains to localize neural elements in 15.5 days post coitus (dpc) fetal mice. To identify potential regulatory factors expressed in pelvic ganglia, we surveyed expression patterns for known or probable transcription factors (TF) annotated in the mouse genome by screenin..
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Awarded by Vanderbilt Ingram Cancer Center
Awarded by Vanderbilt Digestive Disease Research Center
Awarded by NIH
Awarded by US National Institutes of Health
Awarded by National Institutes of Health
Funding Acknowledgements
The authors thank Kevin Weller, David Flaherty, and Brittany Matlock for support in the Flow Cytometry Shared Resource at Vanderbilt University, Stephanie Skelton and Dennis Buehler for assistance with RNA preparation, and Jean-Marc DeKeyser for AChE staining. We appreciate the assistance of Susan Lloyd-MacGilp in the GUDMAP Editorial Office with submission of annotated image files. The VUMC Flow Cytometry Shared Resource is supported by the Vanderbilt Ingram Cancer Center (P30 CA68485) and the Vanderbilt Digestive Disease Research Center (P30 DK058404). Confocal imaging was performed through the use of the VUMC Cell Imaging Shared Resource, which is supported by NIH grants CA68485, DK20593, P30DK058404, HD15052, DK59637, and EY08126. The 2H3 Neurofilament antibody developed by T. M. lessen and J. Dodd was obtained from the Developmental Studies Hybridoma Bank developed by the NICHD and maintained by The University of Iowa, Department of Biological Sciences. Work in EMS<SUP>2</SUP>'s laboratory was supported by funding from US National Institutes of Health grants DK070219 (subcontract), R01-DK078158, and RC1-DK086594. Work in APM's laboratory was supported by a grant from the National Institutes of Health (DK 070181). Work in MHL's laboratory was supported a grant from the National Institutes of Health (DK070136). M. L. is a Principal Research Fellow of the National Health and Medical Research Council of Australia.