New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells
Carmel M O'Brien, Hun S Chy, Qi Zhou, Shiri Blumenfeld, Jack W Lambshead, Xiaodong Liu, Joshua Kie, Bianca D Capaldo, Tung-Liang Chung, Timothy E Adams, Phan Tram, John D Bentley, William J McKinstry, Karen Oliva, Paul J McMurrick, Yu-Chieh Wang, Fernando J Rossello, Geoffrey J Lindeman, Di Chen, Thierry Jarde Show all
STEM CELLS | WILEY | Published : 2017
The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protei..View full abstract
Awarded by State Government of Victoria
Awarded by Australia's National Health and Medical Research Council
Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
This research was supported by California Institute for Regenerative Medicine (CIRM) joint grant funding with the State Government of Victoria (TR1-01250 to A.L.L. and J.F.L.) and Australia's National Health and Medical Research Council (TR3-05603 to C.M.O'B., A.L.L. and J.F.L.). A.L.L. was a Partner Investigator on the Australian Research Council (ARC) Special Research Initiative in Stem Cell Science, Stem Cells Australia. J.E.V. is the recipient of an NHMRC Australia fellowship. We thank personnel at Monash Antibody Technology Facility, Flowcore and Stemcore facilities (Monash University), the CSIRO protein production facility, Anastasia Lukianchikov (UCLA) and WEHI Flow Cytometry for advice and/or technical assistance. We thank surgeons, pathologists and staff at the Victorian Cancer Biobank and Cabrini Hospitals for coordinating the donation of surgery tissues. Prof. Ernst Wolvetang (University of Queensland) kindly provided the hiPS-NHF-1.3 cell line for this study. Prof. David Haylock (CSIRO) kindly provided advice for the analyses of hBM-MSC cultures and Emma Nolan and Francois Vaillant provided expert help with breast tissue preparation. GCTM-2 and CD9 antibodies kindly provided by Prof. Martin Pera (University of Melbourne). S. B. is currently affiliated with Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa Israel; Y.-C. W. is currently affiliated with Department of Pharmaceutical Sciences, The University of North Texas Health Science Center, FortWorth, TX.