Disruption of Serinc1, which facilitates serine-derived lipid synthesis, fails to alter macrophage function, lymphocyte proliferation or autoimmune disease susceptibility
Edward PF Chu, Colleen M Elso, Abigail H Pollock, May A Alsayb, Leanne Mackin, Helen E Thomas, Thomas WH Kay, Pablo A Silveira, Ashley S Mansell, Katharina Gaus, Thomas C Brodnicki
MOLECULAR IMMUNOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017
During immune cell activation, serine-derived lipids such as phosphatidylserine and sphingolipids contribute to the formation of protein signaling complexes within the plasma membrane. Altering lipid composition in the cell membrane can subsequently affect immune cell function and the development of autoimmune disease. Serine incorporator 1 (SERINC1) is a putative carrier protein that facilitates synthesis of serine-derived lipids. To determine if SERINC1 has a role in immune cell function and the development of autoimmunity, we characterized a mouse strain in which a retroviral insertion abolishes expression of the Serinc1 transcript. Expression analyses indicated that the Serinc1 transcrip..View full abstract
Awarded by Australian National Health and Medical Research
We thank Todd Mitchell and Sarah Norris (University of Wollongong, Australia) for assisting with mass spectrometry analysis, Jason Corbin (Walter & Eliza Hall Institute, Parkville, Australia) for technical assistance, and Vicki Moshovakis (St Vincent's Institute, Fitzroy, Australia) for assistance with animal husbandry, and Stuart Mannering (St Vincent's Institute, Fitzroy, Australia) for general discussions regarding this work. This work was supported by a Melbourne International Research Scholarship (E.P.F.C), Saudia Arabia Ministry of Higher Education Scholarship (M.A.A.), Australian National Health and Medical Research grants APP1030865 (T.C.B., K.G., C.M.E., P.A.S.), APP1037321 (H.E.T., T.W.H.K.) and APP1022182 (K.G.) the Australian Phenomics Network (supported by the Australian Government through the National Collaborative Research Strategy Program), and the Victorian Operational Infrastructure Support Program.